Coding
Part:BBa_K3788009:Design
Designed by: Rebecca Pagès Group: iGEM21_Aix-Marseille (2021-09-28)
Colicine A: c.1_1113delins GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1355
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1348
Illegal XhoI site found at 1224 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1424
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 694
Illegal SapI.rc site found at 1178
Design Notes
XbaI restriction site was delated.
Source
colA sequence was given by LISM laboratory from CNRS. GFP coding sequence was taken corresponding to Part:BBa_E0040