Coding

Part:BBa_K3788009:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)


Colicine A: c.1_1113delins GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1348
    Illegal XhoI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1424
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694
    Illegal SapI.rc site found at 1178


Design Notes

XbaI restriction site was delated.


Source

colA sequence was given by LISM laboratory from CNRS. GFP coding sequence was taken corresponding to Part:BBa_E0040

References